egfp tag polyclonal antibody  (Proteintech)

Journal: Frontiers in Microbiology

Article Title: Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island

doi: 10.3389/fmicb.2019.02099

Figure Lengend Snippet: Primers used in this study.

Article Snippet: Immunoblotting, pH 7.6 TBST (150.57 mM NaCl, 24.76 mM Tris base, 0.1% Tween-20) containing 5% skim milk (Difco) for Blocking membrane at 4°C overnight. eGFP rabbit polyclonal antibody (Proteintech, 50430-2-AP) 1:2000 in TBST and membrane was incubated at room temperature for 3 h, and washed 3 times with TBST for 5 min.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island

doi: 10.3389/fmicb.2019.02099

Figure Lengend Snippet: Integrase mediated heterologous gene expression in R. anatipestifer RA-YM strain. (A) The eGFP integration vector construction diagram. (B) Western blot for detecting expression of eGFP protein. The eGFP fluorescent protein were observed by confocal microscope in R. anatipestifer RA-YM wild strain and recombinant strain of integrating eGFP expression (C) .

Article Snippet: Immunoblotting, pH 7.6 TBST (150.57 mM NaCl, 24.76 mM Tris base, 0.1% Tween-20) containing 5% skim milk (Difco) for Blocking membrane at 4°C overnight. eGFP rabbit polyclonal antibody (Proteintech, 50430-2-AP) 1:2000 in TBST and membrane was incubated at room temperature for 3 h, and washed 3 times with TBST for 5 min.

Techniques: Gene Expression, Plasmid Preparation, Western Blot, Expressing, Microscopy, Recombinant

Journal: FEBS letters

Article Title: Mutational analysis revealed that conservation of hepatitis B virus reverse transcriptase residue 306 (rtP306) is crucial for encapsidation of pregenomic RNA.

doi: 10.1016/j.febslet.2007.01.024

Figure Lengend Snippet: Fig. 2. Replication and encapsidation efficiencies of mutants substituted at rtP306. (A) Southern blotting of intracellular capsid-associated viral replicative intermediates produced from rtP306 mutants. (B) Hybridization analysis of encapsidated pgRNA released in situ from intracellular capsids and detected with the antisense riboprobe. (C) Western blotting analysis using polyclonal anti-HBcAg of intracellular capsids produced from rtP306 mutants. (D) Calculated replication and encapsidation efficiencies of mutants substituted at rtP306. Replication and encapsidation efficiency are defined in Section 2. Samples used for these experiments were derived from the same cell transfection experiment. Scanned grey values are shown below each panel, and the value of wild type strain is arbitrarily set to 1.00. RE, replication efficiency; EE, encapsidation efficiency; ND, non detectable.

Article Snippet: Following standard methods, the levels of input 3·FLAG tagged polymerase or EGFP were detected by Western blotting using mouse-derived monoclonal anti-FLAG antibody (M2, Sigma) or rabbit-derived polyclonal anti-EGFP antibody (New England Biolab), followed by HRP-labeled anti-mouse or anti-rabbit secondary antibodies (Amersham Pharmacia Biotech).

Techniques: Southern Blot, Produced, Hybridization, In Situ, Western Blot, Derivative Assay, Transfection

Journal: FEBS letters

Article Title: Mutational analysis revealed that conservation of hepatitis B virus reverse transcriptase residue 306 (rtP306) is crucial for encapsidation of pregenomic RNA.

doi: 10.1016/j.febslet.2007.01.024

Figure Lengend Snippet: Fig. 3. (A) Schematic diagram showing that the location of rtP306 overlaps the 50 modulator element of HBV enhancer I. Enhancer I (966–1308 nucleotide sequence) denoted as an open rectangle consists of three parts: the 50 modulator element, the core element contributing to HBV replication level and the 30 end overlapping with the X promoter. The binding motifs of liver-specific transcription factors, hepatocyte nuclear factor 3 (HNF3) and hepatocyte nuclear factor 4 (HNF4) are indicated as black boxes. A part of the HBV polymerase ORF, RT and RNaseH, are represented as a grid rectangle. The location of rtP306 is shown as a black star. Enh I, enhancer I. (B) Northern blotting analysis of transcripts of HBV mutants substituted at rtP306. EGFP mRNA in each lane was used for normalization of transfection efficiency and as a loading control.

Article Snippet: Following standard methods, the levels of input 3·FLAG tagged polymerase or EGFP were detected by Western blotting using mouse-derived monoclonal anti-FLAG antibody (M2, Sigma) or rabbit-derived polyclonal anti-EGFP antibody (New England Biolab), followed by HRP-labeled anti-mouse or anti-rabbit secondary antibodies (Amersham Pharmacia Biotech).

Techniques: Sequencing, Binding Assay, Northern Blot, Transfection, Control

Journal: FEBS letters

Article Title: Mutational analysis revealed that conservation of hepatitis B virus reverse transcriptase residue 306 (rtP306) is crucial for encapsidation of pregenomic RNA.

doi: 10.1016/j.febslet.2007.01.024

Figure Lengend Snippet: Fig. 4. Trans-complementation assay of rtW58* by the substituted RT mutants. Huh-7 cells were co-transfected with the polymerase depleted replicon rtW58* and eukaryotic expression vectors of 3·FLAG tagged WT-#97 polymerase or its mutants substituted at rtP306. The upper box shows Southern blotting analysis of intracellular viral replicative intermediates; the middle box shows Western blotting analysis of 3·FLAG tagged WT-#97 polymerase or its mutants; the lower box shows expression levels of EGFP used for normalization of transfec- tion efficiency and as a loading control. *, degraded product of EGFP.

Article Snippet: Following standard methods, the levels of input 3·FLAG tagged polymerase or EGFP were detected by Western blotting using mouse-derived monoclonal anti-FLAG antibody (M2, Sigma) or rabbit-derived polyclonal anti-EGFP antibody (New England Biolab), followed by HRP-labeled anti-mouse or anti-rabbit secondary antibodies (Amersham Pharmacia Biotech).

Techniques: Transcomplementation Assay, Transfection, Expressing, Southern Blot, Western Blot, Control